Species 2t. possibly C. calligraphus-group - perhaps species 15 of Wülker.

The identity of this species is quite uncertain.  No larva is known that fits the few criteria listed by Wülker.  It is possible that it is an early designation for the sample from Winter Haven, Florida, since the description and the few images available show a mixture of characters of C. calligraphus and C. species WOC, both of which were found together at this location (see Spies et al. (2002).

Adult
The adults of this species are in the Sublette Collection, now in the museum at the University of Minnesota, St. Paul, MN.

Male:

Male terminalia of C. species 2t (left), and superior volsella (right).

Possibly no setae near middle of tergite IX.  Superior appendage most like Strenzke's S(f)-type.  Anal point narrow; Inferior volsella reaching beyond end of anal point to about the middle of the gonostylus, which is moderately swollen and narrows markedly over the posterior third.
The narrow anal point suggests it may be closer to C. hawaiiensis than C. calligraphus.

Pupa: No information.

Fourth instar larva:  gross morphology not known.  Mentum (below) with pointed teeth; c1 tooth relatively broad with parallel sides, c2 teeth well developed and separated (Ty.III), as that of C. sp. WOC.
Ventromentum (below) with about 35 striae reaching about halfway to the margin, VMR about 0.13.
No information available to me on other mouthparts.

Mentum and ventromentum of C. species 2t.

Cytology:  4 polytene chromosomes with pseudothummi arm combination AE, BF, CD, G.
Arm G (below) paired with nucleolus about a third from one end; two to three Balbiani rings, the positions of which vary due to inversion polymorphism, very similar to that of C. calligraphus.  Centromeres of long chromosomes sometimes inflated like a nucleolus.
Arm F1:    1 - 6b, 19 - 18, 11f - 14, 17 - 15, 11e - 6c, 20 - 23                i.e. as calligraphus & Sp. WOC.
Arm G1:    subterminal nucleolus, with an obvious Balbiani ring towards the other end.  Similar to that of C. calligraphus.

Distal end of arm F (i.e. 1-6b at right end) (above), and arm G (below) of C. species 2t.
Both are consistent with C. calligraphus.

Found: Florida – Winter Haven and Cannon Lake, Polk Co.

Corresponds to species 15 of Wülker.  He noted the existence of polymorphism in arm G, but did not indicate the nature or extent of this polymorphism in the Winter Haven material.  It may reflect the difference between the G of C. calligraphus and C. sp. WOC.