Species 3r. C. species WOC of Wülker & Morath (1989)

Fourth instar larva: a medium plumosus-type larva; length (female) about 11.8mm (Ventral head length abt. 320-340 µm).  Lateral tubules well developed, about 720 µm.  Ventral tubules long, posterior pair longer and coiled (ant. 3.72, post 5.1 mm) and may be over 40% of the larval length; Anal tubules long, 600-740 µm, about 3.3-3.6 times longer than wide (dorsal) to 5.7 (ventral).
Clypeal aperture about 4.5 times longer than wide.  Gula dark to very dark on posterior third to half, frontoclypeus slightly dark to dark, some darkening elsewhere on dorsal head.
Mentum (fig. c) with pointed teeth;  c1 tooth relatively broad with parallel sides, c2 teeth well developed and seperated (type III), 4th laterals reduced to about the level of 5th laterals (type II).
Ventromentum (fig. d) with smooth anterior margin; about 4 times wider than deep; 38-41 striae; VMR about 0.25; Inter Plate Distance about 28-32% mentum width.  Pecten epipharyngis (fig. a) with about 13-16 sharp teeth.
Premandible (fig. b) of type B2 with moderately broad sharp teeth, the inner tooth about 2-3.3 times wider than outer tooth.
Antenna (fig. e) with relatively long basal segment, abt. 4.25 times longer than A2 and abt. 3.5 times longer than wide; Ring organ abt. a third to half way up from base; AR about 2.06; A3 shorter than A5; segment proportions (µm): 135 : 32 : 6 : 14 : 9.
Distance between antennal bases slightly larger than that between S4 setae.
Mandible (fig. f) with third inner tooth fully developed and separated, and darkened (type IIIB-C); about 13-15 furrows on outer surface at base, pecten mandibularis with about 10 -11 taeniae; MTR abt0.38.

Cytology:  4 moderately long polytene chromosomes with pseudothummi arm combination: AE, BF, CD, G.  Arm G short, with a terminal nucleolus and a large Balbiani ring near the other end.  No nucleoli in the long chromosomes.  Centromeres moderately heterochromatic.  No polymorphism known in the small sample of specimens.
WOC A1:    1a-e 9a-e 2d-3b 7d-4a 3i 13a-15e 3h-c 1f-k 8d-g 8a-c 2a-c 10a-12c 16a-19f
WOC B1:    typical bands, groups 25-28, at the centromere
WOC C1:    typical bands, groups 3 and 4, about the middle of the arm
WOC D1:   
WOC E1:    1 - 10b, 12b - 10c, 12c - 13
WOC F1:    1a - 6b, 19 - 18, 11f - 14h, 17 - 15, 11e - 6c, 20 - 23        i.e. as in calligraphus
WOC G1:    Closely paired with a nucleolus at one end and a BR near the other end.

Found: Ontario – Aylmer (42.7706°N, -80.992°W) (GenBank & BOLD)
            Arkansas – (BOLD)            Florida – Winter Haven, Polk Co.;
            Georgia – Camden subdivision, n. Athens, Clarke Co. (33.95°N; 83.37°W).
            Louisiana – Many, Sabine Co.
            Tennessee – White Oak Creek, (abt. 38.2°N; -87.5°W) Knox Co.

Sequences of arms A, E and F given in Wülker & Morath (1989) and photographs of chromosomes in Spies et al. (2002).
Shows affinities to C. anonymus (species 2o), C. calligraphus (species 2w) and to a group of South American species.

Molecular sequence:
mtCOI: There is sequence of an apparently identical species in the BOLD database, but since it is "Private" the Bin no. cannot be determined, but may be the same specimen as in Genbank.