Species 4y C. sp. TE11. (from BOLD database)

In BOLD Bin: BOLD:ADL4581
Some specimens identified as C. lugubris. The nearest neighbor Bin in the BOLD database is BOLD:ADL5630
which is also identified as C. species TE11

Adult:

Male
Some specimens in the BOLD database are almost completely dark brown-black, while others are much lighter, as indicated below.
AR about 3.5-4
Legs - LR about 1.52-1.57; femurs yellowish, knees darkened; Anterior femur and tarsal segments dark; other femurs yellowish, with darkened tarsi.

Abdominal tergites largely dark with a narrow yellowish band at posterior margin.  About 6-11 setae near centre of tergite IX in 3-5 discrete patches.
Superior volsella closest to
Strenzke's S(c), but end quite pointed with a ridge making it appear like the bill of a bird.  Inferior volsella reacing about to end of anal point or about 1/3 of gonostylus.  Characters of anal point not observable.  Gonostylus slender, tapering over posterior third.

Female:
Antennal proportions (percentage of neck in brackets): 26 : 14 (57) : 14 (57) : 13 (7) : 28.  AR about 0.42, A5/A1 about 1.08
Palpal proportions (segs. 2-5): 8 : 30 : 30 : 52 ; P5/P4 about 1.73.
Cercus with anterior margin longer, ventral margin essentially straight with rounded ends, no evident bulge on anterior margin.
(From BOLD specimen MIDGE245-06 identified as C. lugubris).

Pupa not known.

Fourth instar larva:  (from a single larva) A medium sized thummi-type larva, length of female about 14 mm; anterior ventral tubules longer than posterior pair (len 1.24 cf. 1.18 mm).  Gula dark on posterior 1/3 for abt width of mentum, frontoclypeus very slightly dark, with slight darkening elsewhere.  Anal tubules abt 410 x 180 µm (2.3 times longer than wide).  Salivary reservoir 63 x 23 µm (2.8 times longer than wide).
Ventral head length 331 µm; mentum width 170 µm, 0.51 of VHL.  Mentum with 4th laterals slightly reduced (Ty. I-II); center tooth either type IIA or worn III. Ventromental plates about 197 µm long and 3.5 times wider than deep; 1.16 times the Mentum width; with about 53 striae; IPD 0.49; VMR 0.30-0.32.  Pecten epipharyngis not seen.
Premandible with broad teeth, inner tooth about 4.3 times wider than outer tooth.
Antenna with A1 about 0.41 of VHL; 4.1 times longer than wide; RO about 0.37-0.39 up from base; AR 1.96; A5 0.67 the length of A3; approximate segment lengths (micron) 137 : 38 : 11 : 12.5 : 7.5.  Distance between the antennal bases (159 µm) greater than that between the S4 setae (147 µm) which are separated by O. 77 of the Frontoclypeal width.
Mandibles about 255 µm long; third inner tooth partly separated and colored (type IIB); 16-17 furrows on outer surface near the base; about 11 taeniae in Pecten madibularis; Mdt-Mat abt 30 µm, MTR 0.36.

Cytology:  4 polytene chromosomes with the thummi-arm combination AB, CD, EF, G.
Arm G with a virtually terminal nucleolus and two Balbiani rings (BRs) about a quarter from the other end.  Paired only at nucleolus.  No nucleoli in the other chromosomes.  Centromeres obvious.
Arm A1:   
Arm B1:    Puff (group 7) near distal end of arm.
Arm C1:    Constriction (group 4) about one third from centromere.
Arm D1:   
Arm E1:   
Arm F1:    1-2?, abt 18-23.
Arm G1:    Virtually terminal nucleolus, two BRs about a quarter from other end with only a few bands between them.


Polytene chromosomes of C. species TE11
N - nucleolus; BR - Balbiani rings
.

Ontario sample from shallow pool with leaves and debris

Molecular data
MtCOI: Numerous barcode sequences exist in the BOLD database in BIN BOLD:AAB4581.  Some also in GenBank, e.g. KR438689.

Found: Alberta - Banff Natl. Pk. (51.436°N, 116.188°W); Wood Bison Trail, Elkl Island Natl. Pk. (53.567°N, 112.841°W); Jasper Natl.
            Pk. (53.1925°N, 117.954°W)(all BOLD).
            Ontario - about 4.5 km S. & W. Clarence Creek, Russell Co. (45.50°N, 75.22°W); Kawartha Lakes (44.366°N, 78.478°W)
            (BOLD).
            Manitoba - Churchill (58.772°N, 93.843°W) (Ekrem & Stur, BOLD).
            Saskatchewan - Prince Albert Natl. Pk. (53.907°N, 106.075°W)(BOLD).
            Yukon Territory - Ivvavik National Park (69.162°N, 140.155°W); Kluane Natl. Pk. & Reserve (60.748°N, 137.513°W)
            (both BOLD).
            Also NORWAY.

[ Return to Index | Go to References ]

Modified: 26 December 2022
Access: Unrestricted
Copyright © 2017-2022, Jon Martin.