Species 2z.  C. harpi Sublette, 1991

This species is in BOLD Bin: BOLD:AAJ4275


Described by J.E. Sublette in Wülker, Sublette & Martin (1991).

Male: Ground color of head and thorax yellowish, antennal pedicels, thoracic vittae, postnotum, sternoplueron, postnotum and abdomen blackish brown, but abdominal color seasonally variable with late spring specimens with only a basal dark band and distal yellowish band increasing from 1/3 on TII to 2/3 on TV.  Legs also seasonally variable from all dark to F,Ti and Ta1 pale on all legs.
Wing length 3.20 (2.63-3.67) mm.; VR 1.01; r-m crossvein darkened; 26 (23-41) setae in squamal fringe.
Head: AR 3.88 (3.69-4.32); frontal tubercle 15-31 µm; clypeus 0.75 width of ant. pedicel, with 32 (28-40) setae; palpal proportions (segs 2-5) 55 : 195 : 234 : 234.
Thorax with small but distinct mesonotal tubercle. Setae - 19 (16-20) Acrostichal; 25 (21-30) Dorsolateral; 5 (4-6) Prealar; 1 Supraalar; and 29 (23-39) Scutellar.
Leg ratios: PI 1.39 (1.33-1.43); PII 0.59 (0.57-0.62); PIII 0.72 (0.71-0.73); BR 3.36 (2.2-4.67).

Setae on TIX 4 (2-9); SVo closest to E(g)-type of Strenzke (1959); IVo reaching to about end of anal point; gonostylus quite swollen and narrowing sharply about posterior third.

Female: Coloration as in males but coloration darker, legs blackish.
Wing length 4.17 mm; VR 0.95-0.96; 28-43 setae on squamal fringe in 2-3 rows; wing coloration darker than in male.Head: Frontal tubercles about 31 µm long; antennal proportions (micron): 195 : 140 : 125 : 117 : 265; AR 0.46; A5/A1 1.36. Palpal proportions (segs 2-5): 70 : 211 : 257 : 351.  39 clypeal setae.

Pupa:  Described by J.E. Sublette in Wülker, Sublette & Martin (1991).

Larva a small to medium (male 11.2 mm) plumosus- or thummi-type (may be pH dependent?), with posterior ventral tubules usually slighlty longer (ant. 1.56 mm; post. 1.60 mm) and coiled.  Anal tubules relatively long, ventral pair longer (480,540 µm) and 4.8-5.4 times longer than wide. Gular region slightly darkened, frontoclypeus pale.
Mentum with relativley pointed teeth;  c1 tooth relatively broad with short parallel sides, c2 teeth moderately well seperated.

Cytology:  4 polytene chromosomes with thummi arm combination AB, CD, EF, G.
Arm G usually closely paired with a Balbiani ring about 1/3 from one end, although specimens from South Dakota had a nucleolus immediately distal to the BR.  Nucleolus is near the centromere in arm D.
Polymorphism in arm G near distal end, which homozygous in South Dakota specimens, and instead may be heterozygous for an inversion of about 2/3 of arm G.  Polymorphism for arm F also occurs in South Dakota.

harA1:    1a-e, 8-9, 2d-3, 15-13, 4-7, 3f-i, 12c-10, 2c-1f, 16-19;              from utahensis by In7-8
harB1:    Puff, with distal dark bands (groups 7-8), at distal end of arm, but lacks small BR found in proximal region in utahensis
rearranges c.f. utahensisharD1:
harE1:    1-3e, 5-7c, 10c-12, 3f-4, 10b-7d, 13;                        from utahensis by In12-10c
harF1:    1, 9-4c, 14-13c, 2a-4b, 15-17, 10, 13b-11, 18-23.
harF2:  approx 1, 9-4c, 14-13c, 11-13b, 10, 17-15, 4b-2a, 18-23    (South Dakota)
harG1:    BR about 1/3 from one end
harG2:    inversion near distal end
harG3:    inversion of 2/3 arms from harG2

Found: Ontario - Point Pelee National Park (41.959, -82.518) (NCBI)
            Arkansas - 40 km sw. Little Rock, Saline Co. (Type locality) (plumosus-type)
            Illinois - Bradley's Acid Pit (37.90, -89.53), Jackson Co. (thummi-type)
            New York - 2-2.5 km e. Middleport (43.22, -78.47), Orleans Co. (plumosus-type)
            South Dakota - 3.5 km w., 5 km s. Lake Andes, Charles Mix Co.

            Pools with low pH.

Morphology of all life stages, and cytology described by Wülker, Sublette & Martin (1991).  Some ecological data given by Harp and Campbell (1973), as C. plumosus; Harp and Hubbard (1972), as C. n.sp. and Bates and Stahl (1985), as C. nr. maturus.  The South Dakota population may represent a distinct species, however more samples are required to clarify this.  In the meantime it is assumed that this is just geographic differentiation.

Molecular Data
The Barcode sequence of the mitochondrial COI gene, from a larvae collected along with the paratypes from Bradley's Acid Pit, is available on the BOLD database (CotW024-08).  Further sequences from Pt. Pelee National Park, Ont., Canada are available in NCBI (incl. KJ165251).

See also   C. bifurcatus,  C. sp. b,  C. sp. c,  C. sp. j,C. sp. 2a,  C. sp. 2b,  C. "butleri",  C. decorus Joh.,  C. sp. 3h,  C. sp. 3i,  C. sp. 3j,  C. sp. 3r,  C. blaylocki,  C. mozleyi,  C. winnelli.

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Modified: 20 April 2018
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