Species 3a. C. decorus Johannsen, 1905

C. decorus was originally described by Johannsen (1905), with later additions (e.g. Johannsen, 1937) varying, possibly due to inclusion of other members of the species-group.

See here for original description.

Sublette examined the holotype in the Johannsen collection (Sublette et al. 1998) but did not publish any additional description. However he concluded that the current species best fitted Johannsen's material (Wülker et al. 2009).
This is the species studied by Rothfels and Fairlie (1957).

Townes (1945) listed seven synonyms for this species, however some have subsequently been removed from synonymy and others classed as nomina dubia.
Chironomus anonymus Dyer 1902 - subsequently recognised as a distinct species (see species 2o).
Chironomus attenuatus Walker 1848 - on basis of an adult female.  Townes noted it only as a dark colored member of the C. decorus group, but in 1959 revised it to a valid name.  It became a senior synonym of C. decorus until Sublette & Sublette (1974) classed it as a nomen dubium on the basis that the original description and the fact it was a female, meant it could not be accurately identified.
Chironomus cayugae Tilbury 1913.
Chironomus cristatus Malloch 1915 and Kieffer 1917 - misdeterminations of C. cristatus Fabricius.
Chironomus distinguendus Kieffer 1917.
Chironomus maturus Johannsen 1908 - subsequently recognised as a distinct species (see species d)
Chironomus similis Johnannsen 1905 - on basis of female.

In BOLD Bin: BOLD:AAB7030.

Adult:

Male:
Length 6-7 mm.  Wing length about 3.4-4.0 mm; width about 0.91-0.96 mm; VR about 0.91-1.00.  LR about 1.39-1.60 (Johannsen gives 1.6).
Head: Yellow, antennae and proboscis more or less brownish.  AR about 3.01-3.43; cephalic tubercles about 53-58 µm long, and 1.8-2.6 times longer than wide, clypeus about 0.52-0.70 of diameter of antennal pedicel with 28-44 setae; palpal proportions (micron) 77 : 66 : 218 : 260 : 352 (P5/P4 1.3-1.4).
Thorax greenish yellow, stripes and postnotum testaceous or reddish.
Setae: Acrostichal - at least 10-29; Dorsolateral - 21-33; Prealar - 6-7; Supra-alar - 1-2; Scutellar - about 24-41 (13-18 in posterior row and 11-23 in about two or three anterior rows).
Wing: Scf on brachiolum 3-4.  Setae on squamal fringe 33-36.  Distinct brown cloud over r-m crossvein and a paler spot over FCu.
Legs greenish, with tips of tibiae and all tarsi brownish, completely so on 5th tarsus.  Foretarsus without a beard.
Leg lengths (micron) and proportions:

 
Fe
Ti
Ta1
Ta2
Ta3
Ta4
Ta5
LR
F/T
BR
PI
1433
1355
1963
1022
790
710
313
1.39-1.60
1.03-1.07
1.9-3.0
PII
1535
1402
878
500
363
245
172
0.62-0.64
1.09-1.10
 
PIII
1725
1700
1292
750
540
337
198
0.77-0.80
1.01-1.03
 

Note in PI, Ta4 is about 90% the length of Ta3. Abt 15 SensCh on MTa1; abt 27 om HTa1

The male has saddle shaped darker markings about a third from base of each abdominal segment, heaviest on TII-IV, becoming more extensive although paler on segments VI-VIII, occasionally evanescent on V - these bands are narrower than those of C. bifurcatus or C. blaylocki.  About 4-10 setae in 1-4 pale spots in center of tergite IX.


Hypopygia of C. decorus - two variants of hypopygium; note also the long superior volsella.

Typical C. decorus hypopygium; the superior volsella (between S and E-type of Strenzke 1959), while darkened, is paler and longer than that of C. bifurcatus.  Inferior volsella reaching about to end of the anal point, with setae not forked.  Johannsen illustrates the gonostyle as not markedly swollen in the middle and narrowing gently over the distal third.  Townes also shows a form with a markedly swollen gonostyle which narrows sharply at the distal third, similar to C. bifurcatus.  Both types are found from cytologically confirmed material (i.e. reared from egg masses).

Female:
Johannsen notes that the thorax is more greenish and the abdomen greenish with wide dark bands covering most of the segment.
Additional data are available from females reared from egg masses from Wisconsin:
Wing length about 3.36 (3.24-3.48 mm); width about 0.89 (0.80-1.02) mm, VR about 1.0-1.05 (or 0.95-1.0); 20.6 (18-23 setae in the squamal fringe and 3-4 SCf on brachiolum.
Head with cephalic tubercles about 45 (35-71) µm long and 1.5-3.0x as long as wide.  Antennal segments A2-A3 about equal length but with increasing neck length; proportions (micron) and fraction of neck in brackets: 197 (0.31) : 121 (0.34) : 120 (0.38) : 126 (0.43) : 205; AR 0.36 (0.34-0.38); A5/A1 1.04 (0.96-1.11).
Palpal proportions (micron) 57 : 51 : 175 : 228 : 360, A5/A4 1.6.  Clypeus about 1.5-2.3x the width of the antennal pedicel; about 47.5 (42-55) clypeal setae.
Thoracic setae: acrostichal - 18-21; humeral 3-5 linear; dorsolateral - 33-41 (including the humerals - 35-44); prealar - 7; supra-alar - 1-2; scutellar - 23-36 (in about 3 rows, 15-19 in the posterior row and 8-17 in the 2 anterior rows).
Legs as in male.  Fore Ta4 slightly longer than Ta3.  BR about 1.3-2.1.
Lengths (micron) and proportions:

 
Fe
Ti
Ta1
Ta2
Ta3
Ta4
Ta5
LR
F/T
Ta4/Ti
PI
1374
1175
1863
953
773
783
333
1.56-1.68
1.16-1.35
0.66-0.69
PII
1428
1341
809
426
320
218
168
0.58-0.62
0.99-1.14
- 
PIII
1551
1580
1147
660
507
303
195
0.69-0.76
0.96-0.98
 

Abdomen greenish with a dark band 2/3 to 3/4 of segment.  About 4-5 setae on Segment GcIX and 7-10 on seg X which is crescent-shaped and about 4 times longer than the greatest width.

Pupa:  Length of exuviae about 7.4-10.2 mm (fem. 7.56-9.30; ml. 8.13-10.2)mm; inner margin of wing case about 1.57-1.90 mm long.  Exuvia pale, fine shagreen over most of the abdominal tergites, but only a posterior patch on segment VI.The pupa has a definite secondary tubercle on the cephalic tubercles; primary tubercles about 75-196 µm long and about 1.1-2x longer than wide, with a seta up to 105 µm long arising just before the tip; secondary tubercle variable (may be due to mounting differences in some cases) about 23-90 µm long and 0.4-1.1x times longer than wide.
The respiratory base is slightly kidney shaped, with the basal trunk either kidney shaped or partially constricted in the middle.  Length about 148-165 µm and HR 2.2-3.0.  There is a rough patch just anterior to the respiratory base about 37-90 x 24-80 µm which either includes 2 small setae or they are nearby on the lateral side.
About 81 (71-100) recurved hooks at posterior margin of abdominal segment II, central hooks with 2 small knobs on the top and not as downturned as those at either end; hook row continuous, about 57-84% of width of segment (higher values probably due to folding of the abdominal segments of the exuviae).
Pedes spurii B well developed on segment II, and a large (162-220 µm long) pedes spurii A on segment IV (about 0.21-0.26 of length of segment); other pedes spurii A smaller (about 55-95 µm on seg. V; 56-63 µm on seg VI, often with patches of spines). .  L-setae present on the posterior margin of the preceding segment of the intersegments III/IV and IV/V, maybe upto 70-90 µm long.
Usually 4-5 (range 1-6) closely appressed spines only at the tip of the caudolateral spur of segment VIII (below).  Fringe of anal lobe with about 103 (80-138) taenie, in a double row posteriorly but may become triple at end, on each side.

Fourth instar larva a small to medium sized (female 10.7-13.8 mm; male 9.4-13.1) (12 mm according to Johannsen, 1905) bathophilus- or melanotus-type (93-160 µm).  Anterior pair of ventral tubules slightly longer (ant. 1.30 (0.90-1.72 mm; post. 1.21 (0.80-1.57) mm.  In his original description (see above) Johannsen (1905) does not mention lateral tubules but in Johannsen (1937) the larva is noted to possess lateral tubules.  However this is a polymorphic character, apparently present in more northern populations, but lacking in the more southern populations of Mississippi and New Mexico.
Anal tubules 2-3 times longer than wide, variable in length - shortest in Mississippi, and longest in Ontario and Wisconsin, often with a constriction in the middle.

Gular region very dark over posterior half to two thirds, extending beyond the edges of the mentum, higher at lateral margin and wider about a third from posterior margin; frontoclypeus pale.
Mentum (Fig. d) with relatively sharp teeth; c1 tooth relatively broad with parallel sides;  c2 teeth reasonably well but not completely separated (type IIA-III); 4th laterals reduced down to about height of 5th laterals (type (i)-II).
Oesophageal opening about 56-115 µm wide and 2.5-4.4 times wider than deep.
Ventromental plates (Fig. e) about 3.5 times wider than deep, and separated from each other by 0.37-0.42 of the width of the mentum, with about 34.8 (30-51) striae.  Pecten epipharyngis (Fig. a) with about 13.75 (10-15) teeth of type A or sometimes type B.
Premandible (Fig. b) with the broad inner tooth about 2-4 times wider than the outer tooth which narrows markedly along its length, probably about equal length when not worn.
Antenna (Fig. c) relatively short, about 33-46% of ventral head length;  AR about 2.43 (2.19-2.75);  basal segment about 3.2 (2.6-3.7) times longer than wide, ring organ about a third to a half way up the segment from the base; segment lengths (µm) 121 : 27 : 7 : 11 : 6 .
Distance between antennal bases generally greater than that between the S4 setae, which are separated by about 0.7-0.9 of the width of the frontoclypeus at that point.
Mandible (Fig. f) with the third inner tooth slightly darkened and sometimes slightly separated (type I-IIB), about 16.6 (12-20) furrows on outer surface near base; 10.7 (8-13) taeniae in Pecten mandibularis; Mdt-Mat 32.3 (25-38), MTR 0.38 (0.29-0.48).
Larvae from southern regions are bathophilus-type, while those from Wisconsin and Ontario seem to be semireductus- or even plumosus-type.

Cytology:  4 polytene chomosomes (arms A-D or arms E-G) with the thummi arm combination AB, CD, EF, G.  Very polymorphic with inversions in all arms.
Arm G with an a nucleolus near one end - Rothfels and Fairlie (1957) indicate it is in a region about 10 bands from the end.  However the whole region at the end may appear heterochromatic (see figure below) with no nucleolus obvious.  There are three BRs whose relative position depends on the inversion sequence present.  Arm G is often only paired at end away from nucleolus.  No nucleolus in the long chromsomes.
Chromosome AB is somewhat difficult to recognise from the Keyl pattern, since the "olive" in arm A is not obvious and the 4 characteristic bands of arm B are not near the centromere but in variable positions due to inversion polymorphism.  A large puff is sometimes developed in group 7, about one third from the end of arm B.
Polymorphism occurs for all arms.  Rothfels and Fairlie, in their study of 354 individuals, recorded about 30 inversions, including most of those in our smaller studies, with the exception that they found only one sequence for arm F in their Ontario populations, while at least 6 inversions were present in our New Mexico samples.  The inversions from our samples are listed below, with comparison to the R & F sequences where possible.

decA1:    Pattern difficult to identify as the bands of the typical "olive" are dispersed.
decA2:    Large medial inversion.
decA3:    A distal inversion of A2.    May correspond to inversion A of R & F.
decA4:    A small medial inversion.
decB1:    Characteristic bands 20-23 not near centromere but about the middle of the arm, puff (gp. 7) towards distal end of arm.
decB2:    Inversion of about half of the arm, with proximal breakpoint only 10-12 bands from the centromere.
decB3:    Small inversion just proximal to the puff.
decB4:    Small inversion adjacent to the centromere.
decB5:    Small inversion of the more distal region of B2 and apparently sharing the distal breakpoint.
decC1:    Typical groups 3-4 towards the distal end.
decC2:    A very large inversion of about 60% of the arm, with one break distal of the typical bands; as inversion B of R & F.
decD1:    Band groups 16-18 probably towards the distal end of the arm.
decD2:    Large inversion of about 2/3 of arm, proximal breakpoint probably in group 23.
decD3:    Large inversion derived from decD2
decE1:    1 - 3e, 8 - 5, 9 - 10b, 4 - 3f, 10c - 13                      i.e. as maturus, stigmaterus and Inv. A of R & F.
decE2:    1 - 3e, 8i-e, 3f - 4, 10b - 9, 5 - 8d, 10c - 13            i.e. as Inv. S of R & F.
decE3:    appox. 1a-d, 4-3f, 8e-i, 3e-a, 1i-e, 10b-9, 5-8d, 10c-13
decF1:    1, 9 - 2, 10 - 23                                                i.e. as blaylocki, utahensis, etc.
decF2:    1, 3d - 9, 3c - 2, 10 - 23
decF3:    1a-d, 9 - 3d, 1i-e, 3c - 2, 10 - 23(?)                      from F2
decF4:    1, 3d - 9, 3c - 2d, 18 -10, 2a-c, 19 - 23          from F2
decF5:    1, 3d - 9, 17 - 10, 2 - 3c, 18 - 23                         from F2
decF6:    1a-d, 9 - 3d, 1i-e, 3c-2, 10 - 18, 23 - 19              from F3?
decF3+4:    1a-d, 9 - 3d, 1i-e, 3c - 2d, 18 - 10, 2a-c, 19 - 23
decG1:    Subterminal nucleolus and three BR from about 1/3 from nucleolar end to 1/3 from other end, usually only paired at distal end.
decG2:    A small inversion of mid region to just distal of middle BR.
decG3:    A larger complex inversion of almost half the arm, towards the distal end, illustrated in Fig. 1(11) of Rothfels and Fairlie (1957).

Type locality: Johannsen (1905) does not give a type locality but, while noting it is widespread, initially states that it is common in ponds and ditches and, later, in the vicinity of sewage contaminated streams, around Ithaca, New York (1905, 1937).  Townes (1945) lists all localities found in the Johannsen collection as type localities (Noted there as "Johannsen1905" although this is not to imply that they are all this species).
Found: Ontario - Copanspin Farm, Dunrobin (45.75°N, 75.87°W), and South March nr. Mud Lake (44.88°N, 78.27°W), Carleton Co.;
          Don River, Toronto (Rothfels & Fairlie 1957).
          Arkansas - White River National Wildlife Refuge, Arkansas (Chordas et al. 2004.)
          Illinois - (Johannsen 1905).
          Iowa - (Johannsen 1905).
          Kansas - (Johannsen 1905).
          Michigan - Menominee River, Stephenson (45.42°N, 87.61°W), Menominee Co.
          Nebraska - (Johannsen 1905)          New Mexico - Eagle Nest Lake (36.55°N, 105.25°W), Canadian River, Taylor Springs, both Colfax Co.; Rio Grande
                              River, Dona Ana Co.; Hill Tank, Eddy Co.; Pecos River, Puerto de Luna,
                              Guardalupe Co.; Gila River, nr. Virden, Hidalgo Co.; Rio Hondo, 2 ml e. Hondo (33.38°N, 105.26°W), Lincoln Co.
          New York - Ithaca, Tompkins Co. (Johannsen 1905, 1937)
          Ohio - (Johannsen 1905).
          South Dakota - L. Poinsett, 12 ml n. Arlington; James River, Yankton, Yankton Co.
          Washington - (Johannsen 1905)
          Wisconsin - Murphys Creek; and Arboretum, (43.03°N, 89.42°W), and University Houses (43.07°N, 89.42°W), Madison, Dane Co.

Windmill tanks, ponds, ditches, pools in rivers, shallows of lakes.  In some localities it occurs along with C. bifurcatus.  At least where this occurs, the larvae of the two species can be separated by the greater region of gula darkening and the longer anal tubules of C. decorus.

Note that the data for adult females and the pupae all comes from a single reared egg mass, there is considerable variation in some characters mostly these characters are related to overall size of the individual.

Molecular data: (MtCOI) Barcode sequence shows that this species is very close to C. "butleri" (sp. 2g), as both are placed in BOLD Bin AAB7030, as also is C. quinnitukqut.  The sequence for a confirmed C. decorus (sp. 3a) is available and indicates that the sequences differ by only two adjacent bases.
The only certain way to separate them is from the polytene chromosome sequences or from the pupal cephalic tubercles, and possibly in the larva by the greater extent of darkening of the gular region.

Some information on arm F is given in Fig. 3 of Martin (1979) and Wülker, Devai & Devai (1989).  The karyotype was described in some detail in a Report to New Mexico Energy Institute by Martin, Sublette and Sublette (1979).  Also the chromosomes and chromosome polymorphism was described by Rothfels and Fairlie (1957) (see link below).

See also C. bifurcatus,  C. sp. b,  C. sp. c,  C. sp. j,  C. sp. 2a,  C. sp. 2b,  C. 'butleri',  C. sp. 2n,  C. decorus of Rothfels and Fairlie (1957) (see also above);  C. sp. 3h,  C. sp. 3i,  C. sp. 3j,    C. sp. 3r,  C. harpi,  C. blaylocki,  C. mozleyi,  C. winnelli,  C. sp. 4l,  C. sp. 4k.

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Modified: 26 July 2021
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